Rahul Sharma (Editor)

Aspergillus niger

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Scientific name
Higher classification

A fruiting body of Aspergillus

Aspergillus flavus, Penicillium, Aspergillus oryzae, Rhizopus, Trichoderma

Ear fungus microscopic removal aspergillus niger

Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus.


It causes a disease called black mould on certain fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food. It is ubiquitous in soil and is commonly reported from indoor environments, where its black colonies can be confused with those of Stachybotrys (species of which have also been called "black mould").

Aspergillus niger Mould Compendium Aspergillus niger INSPQ Institut national de

Some strains of A. niger have been reported to produce potent mycotoxins called ochratoxins; other sources disagree, claiming this report is based upon misidentification of the fungal species. Recent evidence suggests some true A. niger strains do produce ochratoxin A. It also produces the isoflavone orobol.

An onion infected by Aspergillus niger

Onion infected by aspergillus niger in time lapse number 2


Growth of Aspergillus niger

A. niger is included in Aspergillus subgenus Circumdati, section Nigri. The section Nigri includes 15 related black-spored species that may be confused with A. niger, including A. tubingensis, A. foetidus, A. carbonarius, and A. awamori. A number of morphologically similar species were recently described by Samson et al.

Otomycosis as seen in a microscope.

Recently the strain of ATCC 16404 Aspergillus niger has been reclassified at Aspergillus brasiliensis (refer to publication by Varga et al.). This has required an update to the U.S. Pharmacopoeia and the European Pharmacopoeia which commonly use this strain throughout the pharmaceutical industry.

Plant disease

A. niger causes black mold of onions and ornamental plants. Infection of onion seedlings by A. niger can become systemic, manifesting only when conditions are conducive. A. niger causes a common postharvest disease of onions, in which the black conidia can be observed between the scales of the bulb. The fungus also causes disease in peanuts and in grapes.

Human and animal disease

Colony morphology of Aspergillus niger

A. niger is less likely to cause human disease than some other Aspergillus species. In extremely rare instances, humans may become ill, but this is due to a serious lung disease, aspergillosis, that can occur. Aspergillosis is, in particular, frequent among horticultural workers who inhale peat dust, which can be rich in Aspergillus spores. It has been found in the mummies of ancient Egyptian tombs and can be inhaled when they are disturbed.

A. niger is one of the most common causes of otomycosis (fungal ear infections), which can cause pain, temporary hearing loss, and, in severe cases, damage to the ear canal and tympanic membrane.

Industrial uses

Green Aspergillus niger, a common household mold.

A. niger is cultured for the industrial production of many substances. Various strains of A. niger are used in the industrial preparation of citric acid (E330) and gluconic acid (E574) and have been assessed as acceptable for daily intake by the World Health Organisation. A. niger fermentation is "generally recognized as safe" (GRAS) by the United States Food and Drug Administration under the Federal Food, Drug, and Cosmetic Act.

Many useful enzymes are produced using industrial fermentation of A. niger. For example, A. niger glucoamylase is used in the production of high fructose corn syrup, and pectinases are used in cider and wine clarification. Alpha-galactosidase, an enzyme that breaks down certain complex sugars, is a component of Beano and other products that decrease flatulence. Another use for A. niger within the biotechnology industry is in the production of magnetic isotope-containing variants of biological macromolecules for NMR analysis.

A. niger growing from gold mining solution contained cyano metal complexes, such as gold, silver, copper, iron, and zinc. The fungus also plays a role in the solubilization of heavy metal sulfides. Alkali-treated A. niger binds to silver to 10% of dry weight. Silver biosorbtion occurs via stoichiometric exchange with Ca(II) and Mg(II) of the sorbent.

Other uses

A. niger is the main agent in the fermentation of Pu-erh tea, the Okinawan awamori spirit, and some varieties of shochu.

In 2006, it was reported that a secreted RNase produced by A. niger called actibind has antiangiogenic and anticarcinogenic characteristics.

A. niger is also cultured for the extraction of the enzymes glucose oxidase (GO) and Alpha-galactosidase (AGS). Glucose oxidase is used in the design of glucose biosensors, due to its high affinity for β-D-glucose. Alpha-galactosidase can be produced by A. niger fermentation; it is used to hydrolyze alpha 1-6 bonds found in melibiose, raffinose, and stachyose.

Research published in 2006–2008 investigated A. niger prolyl endoprotease (AN-PEP), a microbial-derived prolyl endoprotease which cleaves gluten. This has strong implications in the treatment of coeliac disease or other metabolic gluten sensitivity disease processes. A placebo controlled, double blind study was initiated in December 2008 to determine the efficacy of this enzyme in treating humans with coeliac disease.

In the heyday of the opium trade, chandu opium, which was meant to be smoked, was made by long term fermentation of A. niger and other moulds on raw opium.

A. niger is often used as a challenge organism for cleaning validation studies performed within sterile manufacturing facilities.


The A. niger ATCC 1015 genome was sequenced by the Joint Genome Institute in a collaboration with other institutions.

The genomes of two A. niger strains have been fully sequenced.


Aspergillus niger Wikipedia

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