|Covid-19|March 7–March 9 – Battle of Shimbra Kure: Imam Ahmad ibn Ibrahim al-Ghazi, with 200 men armed with matchlocks, defeats the army of Lebna Dengel, Emperor of Ethiopia.
April 8 – Flensburg Disputation, a debate, attended by Stadtholder Christian of Schleswig-Holstein (later King Christian III of Denmark) between Lutherans (led by Hermann Fast) and the more radical Anabaptists (led by Melchior Hoffman). Johannes Bugenhagen, a close associate of Martin Luther, presides. The Disputation marks the rejection of radical ideas by the Danish Reformation.
April 9 – The Westrogothian rebellion in Sweden.
April 19 – At the Diet of Speyer, a group of rulers (German: Fürst) and independent cities (German: Reichsstadt) protest the reinstatement of the Edict of Worms, beginning the Protestant movement.
April 22 – The Treaty of Zaragoza divides the eastern hemisphere between Spanish and Portuguese empires, stipulating that the dividing line should lie 297.5 leagues or 17° east of the Moluccas.
May–July – Cardinal Thomas Wolsey, Archbishop of York, presides over a legatine court at Blackfriars, London, to rule on the legality of King Henry VIII of England's marriage to Catherine of Aragon.
May 10 – The Turkish army under Suleiman I leaves Constantinople to invade Hungary once again.
June 21 – War of the League of Cognac: Battle of Landriano: French forces in northern Italy are decisively defeated by Spain.
July 30 – The only Continental outbreak of English sweating sickness reaches Lübeck, spreading from there into Schleswig-Holstein in the next few months.
August 5 – Charles V, Holy Roman Emperor, and Francis I of France sign the Treaty of Cambrai, or "Ladies' Peace" in the War of the League of Cognac: Francis abandons his claims in Italy, but is allowed to retain the Duchy of Burgundy. Henry VIII of England accedes on August 27.
September 1 – Sancti Spiritu, the first European settlement in Argentina, is destroyed by local natives.
Buda is recaptured by the invading forces of the Ottoman Empire.
The city of Maracaibo, Venezuela is founded by Ambrosius Ehinger.
September 23 – Siege of Vienna: Vienna is besieged by the Ottoman forces of Suleiman the Magnificent.
October 15 – With the season growing late, Suleiman abandons the Siege of Vienna, a turning point in the Ottoman wars in Europe.
October 26 – Cardinal Wolsey falls from power in England due to his failure to prevent Habsburg expansion in Europe and obtain an annulment of Henry VIII's marriage. Thomas More succeeds him as Lord Chancellor.
November 4–December 17 – First sitting of the English Reformation Parliament.
Aylesbury is granted the county town of Buckinghamshire in England by King Henry VIII.
Stephen Báthory becomes governor of Transylvania.
Boromrajathira IV succeeds Rama Thibodi II as king of Ayutthaya.
Fluorite is first described by Georg Agricola.
Giorgio Vasari visits Rome.
Pietro Bembo becomes historiographer of Venice.
Heinrich Bullinger becomes pastor of Bremgarten, Switzerland.
Paracelsus visits Nuremberg.
Paracelsus uses the name Paracelsus for the first time.
Occultist Heinrich Cornelius Agrippa publishes Declamatio de nobilitate et praecellentia foeminei sexus ("Declamation on the Nobility and Preeminence of the Female Sex"), a book pronouncing the theological and moral superiority of women.
25 March — Blood libel against the Jewish community of Bosen (formerly in Hungary, today in Slovakia), on the first day of Passover. Three Jews are accused and killed, while the boy is discovered alive, kidnapped for the benefit of the scheme.
January 8 – John Frederick II, Duke of Saxony (d. 1595)
January 13 – Ebba Månsdotter, Swedish noble (d. 1609)
February 14 – Markus Fugger, German businessman (d. 1597)
February 23 – Onofrio Panvinio, Augustinian historian (d. 1568)
April 3 – Michael Neander, German mathematician and historian (d. 1581)
April 25 – Francesco Patrizi, Italian philosopher and scientist (d. 1597)
May 12 – Sabina of Brandenburg-Ansbach, German princess (d. 1575)
June 7 – Étienne Pasquier, French lawyer, poet and author (d. 1615)
June 14 – Ferdinand II, Archduke of Austria, regent of Tyrol and Further Austria (d. 1595)
July 16 – Petrus Peckius the Elder, Jurist, writer on international maritime law (d. 1589)
July 24 – Charles II, Margrave of Baden-Durlach (d. 1577)
August 10 – Ernst Vögelin, Publisher (d. 1589)
September 1 – Taddeo Zuccari, Italian painter (d. 1566)
September 25 – Günther XLI, Count of Schwarzburg-Arnstadt (d. 1583)
October 26 – Anna of Hesse, Countess Palatine of Zweibrücken (d. 1591)
December 11 – Fulvio Orsini, humanist historian (d. 1600)
December 16 – Laurent Joubert, French physician (d. 1582)
Titu Cusi, Inca ruler of Vilcabamba (d. 1571)
Giambologna, Italian sculptor (d. 1608)
Henry Sidney, lord deputy of Ireland (d. 1586)
Michał Wiśniowiecki, prince at Wiśniowiec (d. 1584)
Taddeo Zuccari, Italian painter (d. 1566)
George Puttenham, critic (d. 1590)
January 7 – Peter Vischer the Elder, German sculptor (b. 1455)
January 9 – Wang Yangming, Chinese Neo-Confucian scholar (b. 1472)
February 2 – Baldassare Castiglione, Italian writer and diplomat (b. 1478)
February 4 – Ludwig Haetzer, German Protestant reformer (executed) (b. 1500)
April 20 – Silvio Passerini, cardinal and lord of Florence (b. 1469)
June 21 – John Skelton, English poet (b. c. 1460)
September 6 – George Blaurock, Swiss founder of the Anabaptist Church (b. 1491)
November 20 – Karl von Miltitz, papal nuncio (b. c. 1490)
Krishnadevaraya, Vijaynagar emperor
Richard Pynson, printer (b. 1448)
Andrea Sansovino, Italian sculptor (b. 1467)
Petrus Särkilahti, Finnish Lutheran and scientist
Paulus Aemilius Veronensis, Italian historian (b. 1455)
probable – Lo Spagna, Italian painter
possible – La Malinche, interpreter and translator for Hernán Cortés during the Conquest of Mexico
Year 1529 (MDXXIX) was a common year starting on Friday (link will display the full calendar) of the Julian calendar.