Trisha Shetty (Editor)

Nucleic acid methods

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Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Contents

Purification

  • Phenol–chloroform extraction
  • Minicolumn purification
  • RNA extraction
  • Boom method
  • Synchronous coefficient of drag alteration (SCODA) DNA purification

    • Quantification

  • Abundance in weight: spectroscopic nucleic acid quantitation
  • Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
  • High-throughput relative abundance: DNA microarray
  • High-throughput absolute abundance: serial analysis of gene expression (SAGE)
  • Size: gel electrophoresis

    • Synthesis

  • De novo: oligonucleotide synthesis
  • Amplification: polymerase chain reaction (PCR)

    • Kinetics

  • Multi-parametric surface plasmon resonance
  • Dual-polarization interferometry
  • Quartz crystal microbalance with dissipation monitoring (QCM-D)

    • Gene function

  • RNA interference

    • Other

  • Bisulfite sequencing
  • DNA sequencing
  • Expression cloning
  • Fluorescence in situ hybridization
  • Lab-on-a-chip
  • Comparison of nucleic acid simulation software
  • Northern blot
  • Nuclear run-on assay
  • Radioactivity in the life sciences
  • Southern blot
  • Differential centrifugation (sucrose gradient)
  • Toeprinting assay
  • Several bioinformatics methods, as seen in list of RNA structure prediction software

    • References

    Nucleic acid methods Wikipedia
    (Text) CC BY-SA


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