Neena Gupta was born in Delhi to R. N. Gupta and attended the Lawrence School, Sanawar. She completed her master's degree in Sanskrit and went on to do her M.Phil in the related(?) subject.
Gupta has made appearances in several international films, such as Gandhi (1982), in which she played the niece of Mahatma Gandhi, and the Merchant Ivory films The Deceivers (1988), Mirza Ghalib(1989) In Custody (1993), and Cotton Mary (1999).
She starred along with Madhuri Dixit in Khalnayak (1993); she was featured in the popular song "Choli ke peeche" in the movie. She has made the telefilms Lajwanti and Bazar Sitaram (1993), which won the 1993 National Film Award for Best First Non-Feature Film.
Her big break on television came with Khandaan (1985), Yatra (1986), Gulzar's Mirza Ghalib (1987), a TV miniseries, followed by Shyam Benegal's Bharat Ek Khoj (1988) and later Dard (1994 DD Metro), Gumraah (1995 DD Metro), Saans (Star Plus), Saat Phere: Saloni Ka Safar (2005), Chitthi (2003), Meri Biwi Ka Jawab Nahin (2004), and Kitani Mohabbat Hai (2009).She also acted in one of the famous TV serial Buniyaad.
She also hosted the Indian version of the TV series The Weakest Link, Kamzor Kadii Kaun (Star Plus), and appeared in Jassi Jaissi Koi Nahin (Sony TV), which gave her considerable popularity.
She has directed successful TV series, such as Saans (1999), Siski in (2000) and Kyun Hota Hai Pyarrr. She played the role of Shubha, one of the four women main leads in Ladies Special, a daily soap on Sony TV. She is now seen in Dil se Diya Vachan as Doctor by profession & mother-in-law of the lead character Nandani aired on Zee TV from Monday to Friday at 7pm.
She also ran a theater production company, 'Sahaj Productions' with actor, Rajendra Gupta, and acted and produced Hindi play Soorya Ki Antim Kiran Se Soorya Ki Paheli Kiran Tak.
She has also had some roles in Rishtey which was aired on Zee TV during 1999-2000.
She was in a relationship with former West Indian cricketer Vivian Richards in the 1980s, from which she has a daughter, Masaba Gupta, a fashion designer. She was also involved in relationships with Alok Nath and Sharang Dev. On 15 July 2008, she married New Delhi-based Vivek Mehra, a chartered accountant and Partner with PwC India in a secret ceremony in the United States.National Film Award
1993: Best First Non-Feature Film: Bazaar Sitaram
1994: Best Supporting Actress: Woh Chokri