Rank Species | Genus Medicago Higher classification Medicago | |
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Similar Medicago, Lotus japonicus, Sinorhizobium meliloti, Sinorhizobium, Thale cress | ||
A new crossing technique for medicago truncatula
Medicago truncatula, the barrelclover, strong-spined medick, barrel medic, or barrel medick, is a small annual legume native to the Mediterranean region that is used in genomic research. It is a low-growing, clover-like plant 10–60 cm tall with trifoliate leaves. Each leaflet is rounded, 1–2 cm long, often with a dark spot in the center. The flowers are yellow, produced singly or in a small inflorescence of two to five together; the fruit is a small, spiny pod.
Contents
- A new crossing technique for medicago truncatula
- Medicago truncatula seed germination
- Sequencing of the genome
- Symbioses with soil microorganisms
- References
This species is studied as a model organism for legume biology because it has a small diploid genome, is self-fertile, has a rapid generation time and prolific seed production, is amenable to genetic transformation, and its genome has been sequenced.
It forms symbioses with nitrogen-fixing rhizobia (Sinorhizobium meliloti and Sinorhizobium medicae) and arbuscular mycorrhizal fungi including Rhizophagus irregularis (previously known as Glomus intraradices). The model plant Arabidopsis thaliana does not form either symbiosis, making M. truncatula an important tool for studying these processes.
Medicago truncatula seed germination
Sequencing of the genome
The draft sequence of the genome of M. truncatula cultivar A17 was published in the journal Nature in 2011.
The sequencing was carried out by an international partnership of research laboratories involving researchers from the University of Oklahoma (US), J. Craig Venter Institute (US), Genoscope (France), and Sanger Centre (UK). Partner institutions included the University of Minnesota (US), University of California-Davis (US), the National Center for Genomic Resources (US), John Innes Centre (UK), Institut National de Recherche Agronomique (France), Munich Information Center for Protein Sequences (Germany), Wageningen University (the Netherlands), and Ghent University (Belgium). The Medicago truncatula Sequencing Consortium began in 2001 with a seed grant from the Samuel Roberts Noble Foundation. In 2003, the National Science Foundation and the European Union 6th Framework Programme began providing most of the funding. By 2009, 84% of the genome assembly had been completed.
The assembly of the genome sequence in M. truncatula was based on bacterial artificial chromosomes (BACs). This is the same approach used to sequence the genomes of humans, the fruitfly, Drosophila melanogaster, and the model plant, Arabidopsis thaliana. In July 2013, version 4.0 of the genome was released. This version combined sequences gained from shotgun sequencing with the BAC-based sequence assemblies, which has helped to fill in the gaps in the previously mapped sequences.
A parallel group known as the International Medicago Gene Annotation Group (IMGAG) is responsible for identifying and describing putative gene sequences within the genome sequence.
Symbioses with soil microorganisms
Researcher Toby Kiers of VU University Amsterdam and associates used M. truncatula to study symbioses between plants and fungi – and to see whether the partners in the relationship could distinguish between good and bad traders/suppliers. By using labeled carbon to track the source of nutrient flowing through the arbuscular mycorrhizal system, the researchers have proven that the plants had indeed given more carbon to the more generous fungus species. By restricting the amount of carbon the plants gave to the fungus, the researchers also demonstrated that the fungi did pass along more of their phosphorus to the more generous plants.