Girish Mahajan (Editor)

Lactobacillus sakei

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Domain  Bacteria
Order  Lactobacillales
Genus  Lactobacillus
Higher classification  Lactobacillus
Phylum  Firmicutes
Family  Lactobacillaceae
Scientific name  Lactobacillus sakei
Rank  Species
Lactobacillus sakei Lactobacillus sakei Lacto Bacto
Similar  Lactobacillus, Bacteria, Lactobacillales, Pediococcus, Lactobacillus plantarum

Lactobacillus sakei is a bacterium species in the genus Lactobacillus. It is a facultatively heterofermentative Lactobacillus species (placed in Group II, with species able to produce either alcohol or lactic acid from sugars).

Contents

Lactobacillus sakei Sem Of Lactobacillus Sakei Photograph by Scimat

Uses

Lactobacillus sakei Behavior of the MeatBorne Bacterium Lactobacillus sakei during Its

Antilisterial strains of L. sakei are used in Europe for the production of saucisson and can be used for the conservation of fresh meat.

Lactobacillus sakei strains isolated from traditional dry sausage have a potential use as starter cultures.

Lactobacillus sakei bacmapwishartlabcomsystemimages287mediumLac

Inhibition of Listeria monocytogenes in chicken cold cuts can be obtained by addition of sakacin P and sakacin P-producing Lactobacillus sakei.

Strain 2a of the subspecies Lactobacillus sakei subsp. sakei can also be isolated from meat products.

New research suggests that L. sakei may play a role in maintaining healthy sinus cavities and preventing sinusitis.

Bacteriocins production

Sakacins are bacteriocins of class II produced by L. sakei.

Lactobacillus sakei Lactobacillus sakei Wikipedia

In strain CCUG 42687, their production is dependent on nutrients, temperature and pH. Using the same strain, sakacin P can be produced in a completely defined medium.

In strain CTC 494, the presence of salt and a curing agent (sodium chloride and sodium nitrite) reduces the production of the antilisterial bacteriocin sakacin K. Growth of CTC 494 is also dependent on nutrients availability.

Lactocin S is a bacteriocin produced by strain L45 of Lactobacillus sakei.

Exopolysaccharide biosynthesis

Strain 0–1 of Lactobacillus sakei produces exopolysaccharides.

Prevention

Eugenol is a chemical compound that can be used to reduce the presence of L. sakei as it disrupts its cellular membranes.

Genetics

Genetic diversity within Lactobacillus sakei can be assessed through the use of specifically designed PCR primers for detection using randomly amplified polymorphic DNA.

Bacteriocin genes

Bacteriocin genes are located either on chromosoms or on plasmids. Strain 5 produces a plasmid-encoded bacteriocin that is identical to sakacin P, as well as two chromosomally encoded bacteriocins, which were designated sakacin T and sakacin X.

LasX is a transcriptional regulator of the lactocin S biosynthetic genes in strain L45 of Lactobacillus sakei.

Other genes

In strain LTH677, a starter organism used in meat fermentation, there is an oxygen-dependent regulation of the expression of the catalase gene katA.

In strain LTH681, the stress operon dnaK has been characterized in 1999 as a heat shock protein gene.

There is only one gene (IdhL) responsible for the lactic fermentation.

Plasmids

A Theta-type plasmid has been characterized in Lactobacillus sakei in 2003. It is a potential basis for Low-Copy-Number vectors in Lactobacilli.

Vectors for inducible gene expression in Lactobacillus sakei can be constructed. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone.

Genome

The genome of the meat-borne lactic acid bacterium Lactobacillus sakei 23K has been published in 2005.

It is composed of 1884661 nucleotides forming 1879 protein genes and 84 RNA genes.

References

Lactobacillus sakei Wikipedia


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