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7 Aminoactinomycin D

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Covid-19
Formula  C62H87N13O16
Appearance  Red to dark purple powder
Molar mass  1,270.43 g/mol
7-Aminoactinomycin D 7Aminoactinomycin D Wikipedia

7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It is used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercalates in double-stranded DNA, with a high affinity for GC-rich regions, making it useful for chromosome banding studies.

Contents

Applications

7-Aminoactinomycin D 7Aminoactinomycin D 7AAD Abcam

With an absorption maximum at 546 nm, 7-AAD is efficiently excited using a 543 nm helium–neon laser; it can also be excited with somewhat lower efficiency using a 488 nm or 514 nm argon laser lines. Its emission has a very large Stokes shift with a maximum in the deep red: 647 nm. 7-AAD is therefore compatible with most blue and green fluorophores – and even many red fluorophores – in multicolour applications.

7-Aminoactinomycin D 7Aminoactinomycin D 97 HPLC powder SigmaAldrich

7-AAD does not readily pass through intact cell membranes; if it is to be used as a stain for imaging DNA fluorescence, the cell membrane must be permeabilized or disrupted. This method can be used in combination with formaldehyde fixation of samples.

7-Aminoactinomycin D 7AAD 7Aminoactinomycin D CAS 7240371 ABD17501 AAT Bioquest

7-AAD is also used as a cell viability stain. Cells with compromised membranes will stain with 7-AAD, while live cells with intact cell membranes will remain dark.

Actinomycin D

The related compound actinomycin D is nonfluorescent, but binds DNA in the same way as 7-AAD. Its absorbance changes when bound to DNA, and it can be used as a stain in conventional transmission microscopy.

References

7-Aminoactinomycin D Wikipedia


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