Victor Moore appeared in over 50 films and 21 Broadway shows. His first appearance was on Broadway in Rosemary (1896). He also appeared in George M. Cohan's Forty-five Minutes from Broadway, which opened January 1, 1906, and its sequel, The Talk of New York (1907). He went on to star in shows such as Oh, Kay! (1926) as Shorty McGee, Hold Everything! (1928) as Nosey Bartlett, Gershwin's Of Thee I Sing (1931) as Vice-President Alexander Throttlebottom, Let 'Em Eat Cake (1933), Cole Porter's Anything Goes (1934) as Moonface Martin, and Irving Berlin's Louisiana Purchase (1940) as Oliver P. Loganberry.
Moore made his film debut in 1915. He starred in three films that year, two of which were directed by Cecil B. DeMille – Chimmie Fadden and Chimmie Fadden Out West. He also appeared in Swing Time (1936) with Fred Astaire and Ginger Rogers, Make Way for Tomorrow (1937), The Heat's On with Mae West, Duffy's Tavern (1945), Ziegfeld Follies (1946), It Happened on 5th Avenue (1947), On Our Merry Way (1948), A Kiss in the Dark (1949), and We're Not Married (1952), working with Ginger Rogers for a second time. His last screen appearance was a role as a plumber in The Seven Year Itch (1955).
He worked in film twice with Bob Hope, first in Louisiana Purchase (1941) and again in Star Spangled Rhythm (1942).
Moore made a guest appearance as himself on The Martin and Lewis radio show on August 16, 1949, and was a regular (as himself) on The Jimmy Durante Show.
In 1945 Moore appeared in the Daffy Duck cartoon Ain't That Ducky. He was so pleased with his caricature he offered to add his voice free of charge on the condition that the animators draw him with a little more hair.
He was married twice – first to actress Emma Littlefield from 1902 until her death on June 25, 1934, and then to Shirley Paige in 1942. The marriage was not announced for a year and a half. At the time of the announcement, Moore was 66 years old and Paige was 22. They remained married until Victor Moore's death 20 years later.
He had three children with his first wife: Victor, Jr. (1910), Ora (1919), and Robert (1921).
Moore died of a heart attack on July 23, 1962. He was 86 years old. He is interred at Cypress Hills Cemetery in Brooklyn, New York, United States.
The Victor Moore Bus Terminal and business arcade at the New York City Subway's Roosevelt Avenue / 74th Street station in Jackson Heights, Queens, served by the 7 E F M R trains and the Q33, Q47, and Q49 buses, was named for him when it opened in 1941. In 2005, the arcade was replaced by an Intermodal Transportation Complex serving the same subway and bus lines.