THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interaction, and immunohistochemistry.
Cell Characteristics Growth Information
Note that although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates.
These cells show a large, round, single-cell morphology.
They are derived from the peripheral blood of a 1 year old human male with acute monocytic leukemia. Some of their characteristics are:
This cell line can provide continuous culture, grown in suspension; RPMI 1640 + 10% FBS + 2mM L-Glutamine. The average doubling time is 19 to 50 hours. 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) are also commonly added to inhibit bacterial contamination. Cultures should be maintained at cell densities in the range 2-9x105 cells/ml at 37 °C, 5% CO2. Cells are non-adherent.
Cell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment.