Appearance Crystalline solid ChemSpider ID 24664358 | Pubchem 45379093 | |
 | ||
Phantasmidine is a toxic substance derived from the Ecuadorian poisonous frog Anthony's poison arrow frog (Epipeptobates Anthonyi), more commonly known as the “phantasmal poison frog”. It is a nicotinic agonist, meaning it binds to nicotinic receptors in the body and mimics the effects of the neurotransmitter acetylcholine. This causes the stimulation of the body’s parasympathetic nervous system, which induces many inhibitory behaviors in the body such as decreased heart rate.
Contents
Phantasmidine is characterized in the same class as epibatidine, which is a similar nicotinic acetylcholine agonist derived from a poisonous frog species. Some synthetic processes can even generate phantasmidine from using epibatidine as a starting reagent. Epibatidine and epibatidine-related compounds have an LD50 of around 4 µg in mice; however, the exact LD50 of phantasmidine is not known.
Synthesis
Multiple syntheses of phantasmidine have been proposed, some of which have been included below. The general mechanism for the synthesis of phantasmidine is shown below.
In the general reaction mechanism, 1,2-bis(trimethylsilyloxy)cyclobutene is reacted with the intermediate to form the second product in 85% yield. This is then run under basic conditions to produce an intramolecular aldol reaction followed by an intramolecular nucleophilic aromatic substitution, leading to the lactam product in 46% yield. The final step involves reacting this product with BH3 in THF, then reacting the product with piperazine in MeOH at reflux.
6-chloro-2-fluoro-3-pyridineacetamide is used as an intermediate in most syntheses, which is then reacted through these three general steps to produce phantasmidine. The structure of this intermediate is shown as the starting reagent in the general reaction mechanism above.
Modern synthetic procedures, however, generally begin with 2-chloro-6-fluoropyridine as the starting reagent, due to its commercial availability. This is then converted in several steps to the 6-chloro-2-fluoro-3-pyridineacetamide intermediate. The overall yield of (±) phantasmidine for the modern synthesis shown below is 8% (Zhou, Q. and B. B. Snider 528).
Chemical Properties
(±) Phantasmidine is a crystalline solid. The syntheses of phantasmidine result in racemic mixtures, making it difficult to synthesize an individual enantiomer of phantasmidine. However, through the utilization of HPLC, the individual enantiomers can be separated. HPLC thereby allows for the classification and characterization of (-) and (+) phantasmidine individually.
The assignation for the 1H NMR shift values of (-) phantasmidine are listed below (Fitch et.al.).
The characterized data gathered from the IR spectrum for (-) phantasmidine is shown in the table below (Fitch et al. 17) (Fitch et al. 336-337).
Metabolism
Phantasmidine is a nicotinic agonist that acts at acetylcholine receptors. It mimics the effects of acetylcholine on the body’s neuronal-based nervous systems (both the central nervous system [CNS] and the peripheral nervous system [PNS]) and the muscle-based nervous system (the somatic nervous system).
Mechanism of Action
Nicotinic acetylcholine receptors in general comprise a sub-section of the family of ligand-gated ion channels, of which ions such as Ca+2, Na+, and K+ are permeable to the barrier. Phantasmidine is selective for nicotinic acetylcholine receptors (nAChR) containing ß4 subunits; however, responses in neuromuscular nAChR (such as ß1-containing receptors) and ß2-containing neuronal receptors (such as K-177 cells) are also elicited, albeit to a lesser degree (Fitch et al. 331-337).
Studies conducted in mice have proven useful in showing the interaction and relationship of nAChR α- and ß- subunits to the body’s processes and interaction with this toxin. In particular, α3 and ß4 nAChR subunits have shown to play a role in ganglionic transmission, indicating they play a role in the stimulation of either the parasympathetic nervous system (as an agonist) or the sympathetic nervous system.
The table provided below summarizes the types of cells that phantasmidine affects as well as the resultant behavior exhibited in the listed species. The depolarization of a cell results from the activation of a cation-permeable membrane, which causes an influx of Ca+2 into the cell. This influx of positive charge induces the release of acetylcholine into the body to interact with the parasympathetic nervous system. This, in turn, causes the inhibitory responses exhibited.
Toxicity and Treatment
Not much about the exact toxicity of phantasmidine is known; however, epibatidines in general are 200 times more potent than morphine (Riley 21). Phantasmidine interacts with the body’s stimulation of the parasympathetic nervous system, making it a dangerous inhibitory poison. Symptoms of phantasmidine poisoning may include decreased heart rate, continuous sensations of pain, coma, and (in larger doses) death.
A plausible antidote for phantasmidine poisoning would be mecamylamine, a nAChR antagonist (Zhou 120 – 123). Other nAChR antagonists may serve as effective antidotes for this particular type of poisoning, as they would block nAChR to prevent the acetycholine agonist from binding to the acetylcholine receptors.
Analgesics
Nicotinic agonists in general have proven useful analgesics in treating disorders that respond to nAChR (Fitch et al. 1-17) (Zhou 120-123). For example, phantasmidine or phantasmidine-derivatives are being tested for their potential use as short-acting muscle relaxants. They are also currently being researched as a potential analgesic for the treatment of:
Such treatments can be administered parenterally (intravenously, intradermally, intramuscularly) or through inhalation of a pharmaceutically-composed aerosol.