N,N dimethylformamidase

In enzymology, a N,N-dimethylformamidase (EC 3.5.1.56) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are N,N-dimethylformamide and H₂O, whereas its two products are dimethylamine and formate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N,N-dimethylformamide amidohydrolase. Other names in common use include dimethylformamidase, and DMFase. This enzyme participates in glyoxylate and dicarboxylate metabolism. It employs one cofactor, iron.