Methylguanidinase

In enzymology, a methylguanidinase (EC 3.5.3.16) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are methylguanidine and H₂O, whereas its two products are methylamine and urea.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is methylguanidine amidinohydrolase. This enzyme is also called methylguanidine hydrolase.