Malate dehydrogenase (NAD(P) )

Malate dehydrogenase (NAD(P)⁺) (EC 1.1.1.299, MdH II, NAD(P)⁺-dependent malate dehyrogenase) is an enzyme with systematic name (S)-malate:NAD(P)⁺ oxidoreductase. This enzyme catalyses the following chemical reaction

This enzyme, which was characterized from the methanogenic archaeon Methanobacterium thermoautotrophicum, catalyses only the reduction of oxaloacetate, and can use NAD⁺ and NADP⁺ with similar specific activity. It is different from EC 1.1.1.37 (malate dehydrogenase (NAD⁺)), EC 1.1.1.82 (malate dehydrogenase (NADP⁺)) and EC 1.1.5.4 (malate dehydrogenase (quinone)).