Baby Hamster Kidney fibroblasts (aka BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays occasionally used, subclone 13 which originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.
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BHK and virus infection
BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain). BHK-21 cells are resistant to poliovirus 2. The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes.
Utilization
The BHK-21 cells are useful for transformations and for stable and temporary transfections.
Recommended growth medium
high- glucose DMEM FBS fetal bovine serum 5% (when you freeze, add 10%) GLU glutamine 1% PSA regular antibiotics 1%
Splitting :10 by Trypsin