Rahul Sharma

Aspergillopepsin II

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EC number  3.4.23.19
IntEnz  IntEnz view
ExPASy  NiceZyme view
CAS number  9025-49-4
BRENDA  BRENDA entry
KEGG  KEGG entry
Aspergillopepsin II

Aspergilloglutamic peptidase, also called aspergillopepsin II (EC 3.4.23.19, proctase A, Aspergillus niger acid proteinase A, Aspergillus niger var. macrosporus aspartic proteinase) is a proteolytic enzyme. The enzyme was previously thought be an aspartic protease, but it was later shown to be a glutamic protease with a catalytic Glu residue at the active site, and was therefore renamed aspergilloglutamic peptidase.

Determination of its molecular structure showed it to be a unique two-chain enzyme with a light chain and a heavy chain bound non-covalently with each other. The C-terminal region of the light chain of one molecule binds to the active site cleft of another molecule in the manner of a substrate.

This enzyme catalyses the following chemical reaction

Preferential cleavage in B chain of insulin: Asn3-Gln, Gly13-Ala, Tyr26-Thr

This enzyme is isolated from Aspergillus niger var. macrosporus.

References

Aspergillopepsin II Wikipedia


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