Villanueva started practicing boxing and volleyball. She is a student of Accounting at Universidad Organización y Método and is a member of the Dominican Air Force; there she received the promotion to Sergeant major in 2011.
Ana took the money she collected after the 2011 Pan American Games winning, to repay the mortgage loan over her family house. They used the mortgage to fund the liver cancer treatment for one of Villanueva's brothers.
Villanueva win the gold medal at the team kumite competition during the 2006 Central American and Caribbean Games, held in Colombia.
At the 2009 Central American and Caribbean Championship held in Santiago, Dominican Republic, Ana win the old medal at the Team Kumite category and the silver at the under 50 kg. The next year, she took the gold medal in under 50 kg and bronze in the open category medal at the 2010 Central American and Caribbean Championships
Ana took two bronze medals at the 2010 Central American and Caribbean Games, one in the under 50 kg, and the other at the team kumite competition.
Villanueva won the gold medal in the kumite –50 at the Ibero-American Championship, held in Managua, Nicaragua. Three months later, at the 2011 Pan American Games, Ana win the under 50 kg gold medal, defeating the Chilean Gabriela Bruna in the final match. For this winning she collected a RD$500,000 (approx. US$13,017 in November 2011) award by the President of the Dominican Republic, Mr. Leonel Fernández.
For her achievements during 2011, Ana was selected in February along with Dionicio Gustavo, Karate Athlete of the year. She was also one of the 10 nominees for the Dominican Republic Athlete of the Year, finally won by Luguelín Santos.
In March 2012, her hometown mayor awarded Ana for making them proud for her achievements.
Villanueva won the silver medal of the IV Iberoamerican Championship after losing to Venezuelan Aurimer Campos 3-4. The then won the bronze medal in kumite -50 kg and gold in team kumite.