Ambrose is a surname. Notable people with the surname include:
Alex Ambrose (born 1982), Indian footballerAlice Ambrose (1906–2001), American philosopher, logician, and authorAshley Ambrose (born 1970), former American football playerAutpert Ambrose (c. 730–784), Frankish Benedictine monk who became abbot of San Vicenzo, ItalyCharles Ambrose (1791-1856), English organist, composer, choir director, and music educatorCurtly Ambrose (born 1963), West Indian cricketerDarren Ambrose (born 1984), English footballerDavid Ambrose, British novelist and screenwriterDean Ambrose (born 1985), ring name of American professional wrestler Jonathan GoodDick Ambrose (born 1953), former American football linebackerDonetta W. Ambrose (born 1945), United States federal judgeEddie Ambrose (1894–1994), American jockeyEfe Ambrose (born 1988), Nigerian footballerErin Ambrose (born 1994), Canadian ice hockey playerGladys Ambrose (1930-1998), English film and television actressIsaac Ambrose (1604-1663/64), English Puritan divineJames R. Ambrose (born 1922), former aerospace executive and U.S. Under Secretary of the ArmyJohn Ambrose (c. 1705-1771), Royal Navy officerJohn Thomas Ambrose, former Deputy U.S. Marshal, convicted in 2009 of leaking information about a federal witnessJohn Wolfe Ambrose (1838-1899), Irish-American engineer and developerJ. J. Ambrose (born 1981), American mixed martial artistLauren Ambrose (born 1978), American actressMarcos Ambrose (born 1976), Australian race car driverMyles Ambrose, former U.S. Commissioner of CustomsPaddy Ambrose (1928–2002), Irish footballer and coachPatrick Ambrose (born 1991), Australian rules football playerPaul Ambrose (1868-1941), Canadian organist, conductor, composer, and music educatorRichard Ambrose (born 1972), former Australian rules footballerRob Ambrose (born 1970), American footballer and coachRobert Ambrose (1824-1908), Canadian composerRobert Ambrose, American conductorRona Ambrose (born 1969), Canadian politicianSharon Ambrose (born 1939), U.S. politicianSimon Ambrose, 2007 winner of the BBC reality TV show The Apprentice, chairman of the London Contemporary OrchestraSimon Ambrose, a character from the Johnny English moviesStephen Ambrose (1936-2002), American historianStuart Ambrose (born 1943), former English cricketerTim Ambrose (born 1982), English cricketerTish Ambrose, American pornographic actressTommy Ambrose, Canadian singer-songwriterTony Ambrose (1933-2008), British rally driverWalt Ambrose (1905-unknown), American footballerProfessor Warren Ambrose (1914–1995), American mathematicianWilliam Ambrose (1813–1873), bardic name Emrys, 19th-century Welsh language poetWilliam Ambrose (1832–1908), English judge and politicianJ. Willis Ambrose (1911–1974), first President of the Geological Association of CanadaFictionalDesmond Ambrose, a character on Desmond's