The qualifying round of the 2000–01 UEFA Cup began on 8 August 2000. The round consisted of 41 matches.
Second leg
Pobeda win 2-1 on aggregate
Basel win 12-1 on aggregate
Gorica win 3-2 on aggregate
Rapid Wien win 6-0 on aggregate
Club Brugge win 6-1 on aggregate
Hearts win 5-0 on aggregate
AB win 9-0 on aggregate
Örgryte win 3-1 on aggregate
Košice win 4-2 on aggregate
Napredak Kruševac win 6-2 on aggregate
MTK Budapest win 5-2 on aggregate
Vorskla Poltava win 4-0 on aggregate
Gent win 6-2 on aggregate
Halmstad win 11-0 on aggregate
Vasas win 4-3 on aggregate
Celtic win 11-0 on aggregate
Drnovice win 4-0 on aggregate
APOEL win 5-2 on aggregate
Rapid București win 3-1 on aggregate
Beitar Jerusalem win 4-1 on aggregate
Naftex Burgas win 2-1 on aggregate
Wisła Kraków win 3-1 on aggregate
Olimpija Ljubljana win 3-0 on aggregate
Antalyaspor win 7-0 on aggregate
Ruch Chorzów win 7-2 on aggregate
2-2 on aggregate, Bohemians win on away goals
IFK Norrköping win 4-1 on aggregate
Brann win 2-1 on aggregate
1-1 on aggregate, Maccabi Haifa win on away goals
Slovan Bratislava win 4-0 on aggregate
Partizan win 5-3 on aggregate
CSKA Sofia win 11-2 on aggregate
AIK win 3-0 on aggregate
HJK win 3-2 on aggregate
Lillestrøm win 4-0 on aggregate
Lierse win 7-0 on aggregate
Boavista win 5-0 on aggregate
Rayo Vallecano win 16-0 on aggregate
Lausanne win 2-0 on aggregate
Rijeka win 8-6 on aggregate
Amica Wronki win 6-3 on aggregate