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1 Fluoro 2,4 dinitrobenzene

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Abbreviations  DNFB, FDNB
Melting point  25.8 °C
Appearance  yellow crystals
Density  1.47 g/cm³
Boiling point  296 °C
1-Fluoro-2,4-dinitrobenzene httpsqphecquoracdnnetmainqimgf83533af197e

1-Fluoro-2,4-dinitrobenzene (commonly called Sanger's reagent, dinitrofluorobenzene, DNFB or FDNB) is a chemical used for polypeptide sequencing.

Contents

Preparation

In 1936, Gottlieb presented a synthesis in which 1-chloro-2,4-dinitrobenzene reacted with potassium fluoride (KF) in nitrobenzene:

Uses

In 1945, Frederick Sanger described its use for determining the N-terminal amino acid in polypeptide chains, in particular insulin. Sanger's initial results suggested that insulin was a smaller molecule than previously estimated (molecular weight 12,000), and that it consisted of four chains (two ending in glycine and two ending in phenylalanine), with the chains cross-linked by disulfide bonds. Sanger continued work on insulin, using dinitrofluorobenzene in combination with other techniques, eventually resulted in the complete sequence of insulin (consisting of only two chains, with a molecular weight of 6,000).

Following Sanger's initial report of the reagent, the dinitrofluorobenzene method was widely adopted for studying proteins, until it was superseded by other reagents for terminal analysis (e.g., dansyl chloride and later aminopeptidases and carboxypeptidases) and other general methods for sequence determination (e.g., Edman degradation).

Dinitrofluorobenzene reacts with the amine group in amino acids to produce dinitrophenyl-amino acids. These DNP-amino acids are moderately stable under acid hydrolysis conditions that break peptide bonds. The DNP-amino acids can then be recovered, and the identity of those amino acids can be discovered through chromatography. More recently, Sanger's reagent has also been used for the rather difficult analysis of distinguishing between the reduced and oxidized forms of glutathione and cysteine in biological systems in conjunction with HPLC. This method is so rugged that it can be performed in such complex matrices as blood or cell lysate.

Literature

  • T. Schaefer: "The Proton Magnetic Resonance Spectrum of 1-Fluoro-2,4-dinitrobenzene", Canadian Journal of Chemistry, 1961, 40, pp. 431–433; doi:10.1139/v62-068.
  • B. D. Nageswara Rao: "The 1H and 19F Resonance Spectra of 1-Fluoro-2,4-dinitrobenzene", Molecular Physics, 1964, 7 (4), pp. 307–310; doi:10.1080/00268976300101071.
  • A. Wilkins, R. W. H. Small: "Structure of 1-Fluoro-2,4-dinitrobenzene", Acta Crystallogr., 1991, C47, pp. 220–221; doi:10.1107/S0108270190007326.
  • References

    1-Fluoro-2,4-dinitrobenzene Wikipedia


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