It branches off from St. Mary's Road in north St. Vital and runs southeastward through central and southern St. Vital to the Perimeter Highway. It is a collector road for traffic between south St. Boniface, central and south St. Vital, and downtown. St. Anne's Road from St. Mary's Road to Fermor Avenue is part of the Trans-Canada Highway.
St. Anne's Road continues south of the Perimeter Highway and ends at the Red River Floodway. This part of the road was formerly signed as Provincial Road 300.
The speed limit on Route 150 is 60 km/h (40 mph), except for the section directly north of the Perimeter Highway, where it increases to 70 km/h (45 mph).
Outside of Winnipeg, the road is generally spelled Ste. Anne's Road (French: Chemin Sainte-Anne).
Future
In 2013, the Manitoba government unveiled futures plans to construct an interchange at the junction of the Perimeter and St. Anne's Road, to replace the at-grade intersection and traffic lights that currently exist.