DuBois became a Certified Public Accountant in the 1970s after becoming a silver medalist on his CPA exam. DuBois was offered highest paying job in his class. He went on to obtain his master's and in 2016 he was awarded an honorary PhD in accounting. DuBois moved to Dallas, Texas, where he held positions with Arthur Anderson & Co. and the Federal Reserve Bank of Dallas at 24 years old.
In 1977, DuBois moved to Nashville, Tennessee to foster his interests for songwriting and music. From 1977 to 1985, DuBois worked as both a publishing company staff writer and as an accounting professor at several universities including Vanderbilt University, Tennessee State University, University of Tennessee. Throughout that period, DuBois composed over 20 country singles. Five of those singles, including Alabama's "Love in the First Degree", "The Bluest Eyes in Texas" by Restless Heart and "She Got the Goldmine (I Got the Shaft)" by Jerry Reed, reached No. 1 chart positions.
DuBois opened the Nashville branch of Los Angeles-based artist management firm Fitzgerald-Hartley in 1986. Country singer Vince Gill soon joined the company's inaugural client, Restless Heart. DuBois and Gill collaborated on some songwriting projects including the Country Music Association's 1990 Song of the Year, "When I Call Your Name".
In 1989, Clive Davis, founder of Arista Records, appointed DuBois to open the Nashville division of the label. Arista Nashville sold 80 million albums in its first eleven years of business, breaking acts like Alan Jackson, Brooks & Dunn, Pam Tillis, Diamond Rio, Steve Wariner, and Brad Paisley. DuBois later joined producer Tony Brown to operate Universal South Records, launching the careers of Joe Nichols, Cross Canadian Ragweed, and Shooter Jennings.
In 1991, DuBois married Pamela Smith from Dallas, Texas, a friend he had known for fifteen years. They had a daughter in 1994 and named her after DuBois-James Timothy DuBois: Jamie Grace DuBois and is a very strong example of DuBois.
In 2007, DuBois returned to the faculty of Vanderbilt's Owen Graduate School of Management, where he is developing courses related to the music business. He has also joined forces with Marc Dottore to form Dottore-DuBois Artist Management, dedicated to providing their clients with the tools and guidance to navigate and thrive in the music industry of the 21st century. DuBois resides in Nashville, Tennessee.
In February 2010, ASCAP announced they would elevate their Nashville outlet to a Regional Office, led by DuBois. DuBois holds the position of Vice President and Managing Executive. After restructuring ASCAP, DuBois was asked to join London Broadcast Company in January 2012, based in Dallas, Texas. He started a joint-venture called AMP (Artists, Managers, Partners) which he is still managing.
DuBois serves on the boards of the Country Music Association, Academy of Country Music, Americana Music Association, and SunTrust Bank, and as chairman of the Nashville Convention and Visitors Bureau. He is a past board member of Leadership Music, Country Music Foundation, National Academy of Recording Arts & Sciences, Nashville Songwriters Foundation, and Nashville Songwriters Association International.
Tim DuBois has been recognized as "Record Executive of the Year" by Pollstar, and as one of the entertainment industry's "101 Most Powerful People" by Entertainment Weekly. He was inducted into Oklahoma State University's Hall of Fame in 1996. Throughout his songwriting career, DuBois has earned five number-1 singles, 24 top-ten singles, six ASCAP Awards, nine BMI Country Awards, two BMI Pop Awards and a number of other songwriting accolades.