The movie flips between following a group of gamers in a dorm immersed in a role-playing game, and their characters (played by the same actors) as they journey through a world of fantasy and wonder to defeat a being known as "The Shadow" and rescue a princess. The latter is theoretically a heroic quest, but while the game master attempts to foster in a narrative, the players tend to be more interested in their characters' tolerance for ale, trying to apply the sneak attack damage multiplier to siege weaponry, et cetera.
The movie both makes fun of gamer stereotypes and plays the discrepancy between an optimal RPG plot and the events of an actual RPG session for maximum absurdity. Characters attempting something dangerous freeze as dice clatter in the distance. The party thief picks the pocket of a bystander, then steals that bystander's pants—not because he wants the pants in any way, but solely to see if his skill statistics allow it. When an unlucky dice roll causes the death of one character, the other players' characters mourn him for a moment, and then immediately start squabbling over the items he was carrying. Characters spring to action, then keel over as their players forget and are reminded that the characters are asleep, players argue and make snack runs, and their game is frequently interrupted by a girl from the same dorm who demands they keep quiet so that she can study.
The Shadow is eventually defeated, after a long quest, and the characters find themselves in a strange tunnel. On the other side of the door, they hear voices—the voices of their players, who are narrating the action in the hallway outside. Bursting into the room (as dictated by the players), the characters slaughter the gamers, then begin picking over the room for treasure. Discovering that their entire adventure has been documented by the 'strange sorcerers' they've just slain, they discover their character sheets (which inadvertently insults the group's warrior, because his Intelligence statistic is only 4). In the midst of these revelations, the 'Princess' (the girl who needs to study and is constantly interrupted by the gamers' noise) bursts in and reads them the riot act, thinking their outfits to be mere costumes; after she leaves, the adventurers seem quite stunned that their beloved Princess has such a foul mouth and temper.
In 2013 Zombie Orpheus Entertainment in cooperation with Dead Gentlemen Productions and Lynnvander Inc produced two mini miniseries, "Natural One" and "Humans & Households", set in the Gamers universe. Both series mirror the structure of "The Gamers" by showing both players of a roleplaying game and the fictitious characters they are playing.
"Natural One" features the characters Leo and Gary (both are main characters in "Dorkness Rising" and "Hands of Fate"). The plot revolves around Monica, Gary's sister, who wants to marry her fiancé, Ryan. However, since Ryan is not a geek and an old agreement between Gary and Monica states that both of them may only marry geeks, Ryan has to prove his geekiness by beating a roleplaying game scenario before Gary gives his blessing to the wedding. The fictitious roleplaying game featured in the film is an homage to the popular cyberpunk roleplaying game Shadowrun.
"Humans & Households" (the title is a reference to Dungeons & Dragons) turns the concept of "The Gamers" on its head: Characters in the fantasy world of "The Gamers" play a roleplaying game set in our world.
In April 2015, a web series continuation of the plot of The Gamers was announced. It will lead directly into the events of the next movie in the series and will chronicle exploits of the protagonists' characters who are now lost in the modern world. The project will be funded via Kickstarter, much like The Gamers: Hands of Fate. The first episode of the series dubbed "The Shadow Menace" was released in August 2017.