TRNA (carboxymethyluridine34 5 O) methyltransferase

TRNA (carboxymethyluridine³⁴-5-O)-methyltransferase (EC 2.1.1.229, ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine³⁴-5-O)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + carboxymethyluridine³⁴ in tRNA ⇌ S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine³⁴ in tRNA

The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.