Supriya Ghosh (Editor)

Spermidine

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3DMet
  
B01214

Density
  
925 kg/m³

Formula
  
C7H19N3

Appearance
  
Colourless liquid


Related amines
  
Dipropylamine Methyl-n-amylnitrosamine Norspermidine Dibutylamine Iproheptine

Spermidine is a polyamine compound (C
7H
19N
3) found in ribosomes and living tissues, and having various metabolic functions within organisms. It was originally isolated from semen.

Contents

Function

Polyamines, such as spermidine, are polycationic aliphatic amines and are multifunctional. They serve vital roles in cell survival.

Spermidine synthase (SPDS) catalyzes the formation of spermidine from putrescine. Spermidine is a precursor to further polyamines, such as spermine and its structural isomer thermospermine.

Spermidine synchronizes an array of biological processes (such as Ca2+, Na+, K+ -ATPase) thus maintaining membrane potential and controlling intracellular pH and volume. Spermidine regulates biological processes, such as Ca2+ influx by glutamatergic N-methyl-d-aspartate receptor (NMDA receptor), which has been associated with nitric oxide synthase (NOS) and cGMP/PKG pathway activation and a decrease of Na+,K+-ATPase activity in cerebral cortex synaptosomes.

Spermidine is a longevity agent in mammals due to various mechanisms of action, which are just beginning to be understood. Autophagy is the main mechanism at the molecular level, but evidence has been found for other mechanisms, including inflammation reduction, lipid metabolism, and regulation of cell growth, proliferation and death.

Spermidine is known to regulate plant growth, assisting the in vitro process of transcribing RNA, and inhibition of NOS. Also, spermidine is a precursor to other polyamines, such as spermine and thermospermine, some of which contribute to tolerance against drought and salinity in plants.

Spermidine has been tested and discovered to encourage hair shaft elongation and lengthen hair growth. Spermidine has also been found to “upregulate expression of the epithelial stem cell-associated keratins K15 and K19, and dose-dependently modulated K15 promoter activity in situ and the colony forming efficiency, proliferation and K15 expression of isolated human K15-GFP+ cells in vitro.”

Biochemical actions

Spermidine's known actions include:

  • Inhibits neuronal nitric oxide synthase (nNOS)
  • Binds and precipitates DNA
  • Polyamine plant growth regulator

    • Uses

  • Spermidine can be used in electroporation while transferring the DNA into the cell under the electrical impulse. May be used for purification of DNA-binding proteins.
  • Spermidine is also used, along with calcium chloride, for precipitating DNA onto microprojectiles for bombardment with a gene gun.
  • Spermidine has also been reported to protect the heart from aging and prolong the lifespan of mice, while in humans it was correlated with lower blood pressure. It also was found to reduce the amount of aging in yeast, flies, worms, and human immune cells by inducing autophagy. Recently Tirupathi Pichiah et al., suggested that spermidine may be helpful for treating type 2 diabetes.
  • Spermidine is commonly used for in vitro molecular biology reactions, particularly, in vitro transcription by Phage RNA polymerases, in vitro transcription by human RNA polymerase II (in vitro transcription by human RNA polymerase II, and in vitro translation.
  • Spermidine increases specificity and reproducibility of Taq-mediated PCR by neutralizing and stabilizing the negative charge on DNA phosphate backbone.
  • Spermidine is at physiological pH a polycationic reagent that aids in enzyme digestion by forcing apart DNA molecules.

    • References

    Spermidine Wikipedia
    (Text) CC BY-SA