Formula C27H34I2N4 | ||
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Similar DAPI, Acridine orange, Hoechst stain |
Propidium iodide staining
Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells. Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualise the nucleus and other DNA-containing organelles. Propidium Iodide cannot cross the membrane of live cells, making it useful to differentiate necrotic, apoptotic and healthy cells. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.
Contents
Propidium iodide
References
Propidium iodide Wikipedia(Text) CC BY-SA