Pitrilysin

Pitrilysin (EC 3.4.24.55, Escherichia coli protease III, protease Pi, proteinase Pi, PTR, Escherichia coli metalloproteinase Pi) is an enzyme. This enzyme catalyses the following chemical reaction

Preferential cleavage of -Tyr¹⁶- Leu- and -Phe²⁵- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as glucagon

This enzyme is present in bacteria Escherichia coli.