Supriya Ghosh (Editor)

Phycobiliprotein

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Phycobiliproteins are water-soluble proteins present in cyanobacteria and certain algae (rhodophytes, cryptomonads, glaucocystophytes) that capture light energy, which is then passed on to chlorophylls during photosynthesis. Phycobiliproteins are formed of a complex between proteins and covalently bound phycobilins that act as chromophores (the light-capturing part). They are most important constituents of the phycobilisomes.

Major phycobiliproteins:

Characteristics and applications in biotechnology

Phycobiliproteins elicit great fluorescent properties compared to small organic fluorophores, especially when high sensitivity or multicolor detection is required :

  • Broad and high absorption of light suits many light sources
  • Very intense emission of light: 10-20 times brighter than small organic fluorophores
  • Relative large Stokes shift gives low background, and allows multicolor detections.
  • Excitation and emission spectra do not overlap compared to conventional organic dyes.
  • Can be used in tandem (simultaneous use by FRET) with conventional chromophores (i.e. PE and FITC, or APC and SR101 with the same light source).
  • Fluorescence retention period is longer.
  • Very high water solubility
  • As a result, phycobiliproteins allow very high detection sensitivity, and can be used in various fluorescence based techniques fluorimetric microplate assays, Flow Cytometry, FISH and multicolor detection.

    References

    Phycobiliprotein Wikipedia