Phthalyl amidase

In enzymology, a phthalyl amidase (EC 3.5.1.79) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are phthalylamide and H₂O, whereas its two products are phthalic acid and substituted amine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is phthalyl-amide amidohydrolase.