Phosphomethylpyrimidine synthase

Phosphomethylpyrimidine synthase (EC 4.1.99.17, thiC (gene)) is an enzyme with systematic name 5-amino-1-(5-phospho-D-ribosyl)imidazole formate-lyase (decarboxylating, 4-amino-2-methyl-5-phosphomethylpyrimidine-forming). This enzyme catalyses the following chemical reaction

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine ⇌ 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO

This enzyme binds a 4Fe-4S cluster.