Viewers follow Peep, Chirp, and Quack as they investigate and explore the world around them. Following the 9-minute animated segment, there is a 2-minute live-action segment with children exploring and demonstrating the same topic presented in the animated segment. Animation consists of bright colors and simple shapes.
Peep (Voiced by Scott Beaudin): A male, yellow chicken who is very curious. The show's title is derived from his name. Peep is female in the original short film.
Chirp (Voiced by Amanda Soha): A red, female robin who is also curious, and attempts frequently to fly, with unsatisfying results. She is Peep's friend. Chirp is male in the original short film.
Quack (Voiced by Jamie Watson): A male, blue duck who wears a white sailor's hat. He frequently boasts about himself and ducks as a whole, and unwittingly makes many discoveries. Quack is purple in the original short film
The Narrator (Voiced by Joan Cusack): The Narrator tells the episode's story, although the characters rarely have any interaction with her. In the episode "Dry Duck Part 1" the birds did have a short interaction with her (mainly Quack.)
Ant (Voiced by Robert Tinkler): A busy male ant who appears in some episodes.
Beaver Boy (Voiced by Tessa Marshall): A young naïve male beaver lives in an adjoining pond to Quack's. He appears in several episodes.
Beaver Mom: Beaver Boy's workaholic mother.
Beaver Dad (Voiced by Phillip Williams): Beaver Boy's businesslike father.