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US 1 (or the Overseas Highway) crosses the key at approximately mile marker 39, between Missouri Key and Bahia Honda Key.
Map of Ohio Key, Florida 33043, USA
It was once known as Little Duck Key, however the name Little Duck Key is currently used for a very small island about a mile east that is the western terminus of the Seven Mile Bridge.
Today it is also known as Sunshine Key, after a camping resort located there.
The portion of the island south of U.S. Route 1 is protected as the Ohio Key National Wildlife Refuge.
References
Ohio Key Wikipedia(Text) CC BY-SA