The planet Neptune has been used as a reference and setting in various films and works of fiction:
In H. G. Wells's short story The Star, Neptune is destroyed in a collision with another supermassive object which reduces its orbital velocity to zero; the wreckage falls into the Sun, narrowly missing Earth.
In the Captain Future series, Neptune is portrayed as a sea planet, not out of any scientific theory but evidently because Neptune is the Roman sea god.
In Olaf Stapledon's 1930 epic novel Last and First Men, Neptune is the final home of the highly evolved human race. The planet is depicted as having a dense atmosphere but with a solid surface.
In Hugh Walters' 1968 novel Nearly Neptune, the first manned expedition to Neptune ends in apparent disaster as a fire destroys vital equipment on board the spacecraft as it nears the planet.
In Call of Duty: Infinite Warfare, Neptune is the setting for the multiplayer map, Frontier, which takes place on a space station orbiting the planet.
The planet served as the backdrop for the 1997 science fiction/horror film Event Horizon.
The humorous short story, "The Elephants on Neptune" by Mike Resnick, was published in Asimov's Science Fiction, and was nominated for both a Hugo and a Nebula award (2001).
The pilot of the TV movie Virtuality centers around a starship preparing to make a flyby of Neptune before leaving the solar system.
The Star Trek: Enterprise pilot episode “Broken Bow” briefly mentions Neptune, with Jonathan Archer saying that at Warp 4.5 speed, it is possible to fly to “Neptune and back [to Earth] in six minutes.”
Mothstorm (2008), a book in the Larklight Trilogy by Philip Reeve. Neptune is called Hades. The lizard-like Silth tow their miniature Sun into orbit of it, allowing them to inhabit it and rename it Snil. The remainder of the giant moths they farmed are taken there also.
In the point and click game Anastronaut: The Moon Hopper, the player visits the planet Neptune in a future setting.
In the anime series Sailor Moon, one of the supporting characters is named Sailor Neptune, she is also known as Michiru Kaioh (Michelle in English dub). She fights along with the other Outer Senshi for the Moon Kingdom and protect the Solar System from outside enemies. She carries a talisman known as Deep Aqua Mirror and her powers are based in deep water.
In the cartoon series Futurama, the character Robot Santa Claus has his heavily fortified home base on the north pole of Neptune.
In The Fairly OddParents episode "Wishology! Part 3: The Final Ending," Poof stick wand to Neptune.
The Doctor Who episode "Sleep No More" is set on a space station orbiting Neptune.
In the Hyperdimension Neptunia series, Neptune is the goddess of the nation known as Planeptune.
The french comics Les Fantômes de Neptune (2015), by Valentine Pasche is a steampunk adventure. The stories beggins in Europa, Jupiter's Moon, and it will continue on Neptune.