Nearly neutral theory of molecular evolution

Origins of the nearly neutral theory

According to the neutral theory of molecular evolution, the rate at which molecular changes accumulate between species should be equal to the rate of neutral mutations and hence relatively constant across species. However, this is a per-generation rate. Since larger organisms have longer generation times, the neutral theory predicts that their rate of molecular evolution should be slower. However, molecular evolutionists found that rates of protein evolution were fairly independent of generation time.

Noting that population size is generally inversely proportional to generation time, Tomoko Ohta proposed that if most amino acid substitutions are slightly deleterious, this would increase the rate of effectively neutral mutation rate in small populations, which could offset the effect of long generation times. However, because noncoding DNA substitutions tend to be more neutral, independent of population size, their rate of evolution is correctly predicted to depend on population size / generation time, unlike the rate of non-synonymous changes.

In this case, the faster rate of neutral evolution in proteins expected in small populations (due to a more lenient threshold for purging deleterious mutations) is offset by longer generation times (and vice versa), but in large populations with short generation times, noncoding DNA evolves faster while protein evolution is retarded by selection (which is more significant than drift for large populations) In 1973, Ohta published a short letter in Nature suggesting that a wide variety of molecular evidence supported the theory that most mutation events at the molecular level are slightly deleterious rather than strictly neutral.

Between then and the early 1990s, many studies of molecular evolution used a "shift model" in which the negative effect on the fitness of a population due to deleterious mutations shifts back to an original value when a mutation reaches fixation. In the early 1990s, Ohta developed a "fixed model" that included both beneficial and deleterious mutations, so that no artificial "shift" of overall population fitness was necessary. According to Ohta, however, the nearly neutral theory largely fell out of favor in the late 1980s, because the mathematically simpler neutral theory for the widespread molecular systematics research that flourished after the advent of rapid DNA sequencing. As more detailed systematics studies started to compare the evolution of genome regions subject to strong selection versus weaker selection in the 1990s, the nearly neutral theory and the interaction between selection and drift have once again become an important focus of research.

Theory

The rate of substitution, ρ is

where u is the mutation rate, g is the generation time, and N e is the effective population size. The last term is the probability that a new mutation will become fixed. Early models assumed that u is constant between species, and that g increases with N e . Kimura’s equation for the probability of fixation in a haploid population gives:

where s is the selection coefficient of a mutation. When | s | << 1 N e (completely neutral), P f i x = 1 N e , and when − s >> 1 N e (extremely deleterious), P f i x decreases almost exponentially with N e . Mutations with − s ≃ 1 N e are called nearly neutral mutations. These mutations can fix in small- N e populations through genetic drift. In large- N e populations, these mutations are purged by selection. If nearly neutral mutations are common, then the proportion for which P f i x << 1 N e is dependent on N e

The effect of nearly neutral mutations can depend on fluctuations in s . Early work used a “shift model” in which s can vary between generations but the mean fitness of the population is reset to zero after fixation. This basically assumes the distribution of s is constant (in this sense, the argument in the previous paragraphs can be regarded as based on the “shift model”). This assumption can lead to indefinite improvement or deterioration of protein function. Alternatively, the later “fixed model” fixes the distribution of mutations’ effect on protein function, but allows the mean fitness of population to evolve. This allows the distribution of s to change with the mean fitness of population.

The “fixed model” provides a slightly different explanation for the rate of protein evolution. In large N e populations, advantageous mutations are quickly picked up by selection, increasing the mean fitness of the population. In response, the mutation rate of nearly neutral mutations is reduced because these mutations are restricted to the tail of the distribution of selection coefficients.

The “fixed model” expands the nearly neutral theory. Tachida classified evolution under the “fixed model” based on the product of N e and the variance in the distribution of s : a large product corresponds to adaptive evolution, an intermediate product corresponds to nearly neutral evolution, and a small product corresponds to almost neutral evolution. According to this classification, slightly advantageous mutations can contribute to nearly neutral evolution.

The "drift barrier" theory

Michael Lynch has proposed that variation in the ability to purge slightly deleterious mutations (i.e. variation in N e ) can explain variation in genomic architecture among species, e.g. the size of the genome, or the mutation rate. Specifically, larger populations will have lower mutation rates, more streamlined genomic architectures, and generally more finely tuned adaptations.