Nathusius is a surname. Notable people with the surname include:
Annemarie von Nathusius (1874-1926), German novelist
Hermann von Nathusius (1809–1879), German animal breeder
Johann Gottlob Nathusius (1760–1835), German industrialist
Marie Nathusius (1817-1857), German novelist
Philipp von Nathusius (publisher) (1815-1872), German publisher
Suzanna von Nathusius, Polish child film actor
Thomas von Nathusius (1866–1904), German landscape and animal painter
Susanne von Nathusius (1850–1929), portrait painter