N4 (beta N acetylglucosaminyl) L asparaginase

N4-(beta-N-acetylglucosaminyl)-L-asparaginase (EC 3.5.1.26, aspartylglucosylamine deaspartylase, aspartylglucosylaminase, aspartylglucosaminidase, aspartylglycosylamine amidohydrolase, N-aspartyl-beta-glucosaminidase, glucosylamidase, beta-aspartylglucosylamine amidohydrolase, 4-N-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase) is an enzyme with systematic name N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase. This enzyme catalyses the following chemical reaction

N⁴-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H₂O ⇌ N-acetyl-beta-D-glucosaminylamine + L-aspartate

This enzyme acts only on asparagine-oligosaccharides containing one amino acid.