N substituted formamide deformylase

In enzymology, a N-substituted formamide deformylase (EC 3.5.1.91) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are N-benzylformamide and H₂O, whereas its two products are formate and benzylamine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzylformamide amidohydrolase and is also called NfdA. The enzyme is produced by Arthrobacter pascens bacteria.