Mifune (三船 or 御船) is a Japanese surname. Notable people with the surname include:
Toshirō Mifune (三船 敏郎) (1920 - 1997), Japanese actor who appeared in almost 170 feature films
Jiro Mifune (born 1972), game designer
Mifune Chizuko (御船千鶴子) (1886 - 1911), female clairvoyant
Mika Mifune(三船美佳|三船美佳), actress
Kyuzo Mifune (三船久蔵) (1883 - 1965), judoka
Fictional characters:
Captain Mifune, a character from The Matrix Revolutions
Mifune (Soul Eater), a character in the manga and anime Soul Eater
Admiral Mifune, a character from the anime The Irresponsible Captain Tylor
Go Mifune, main character in the anime series Speed Racer
Lord Mifune, a feudal ruler in Usagi Yojimbo
General Mifune, a Samurai general in Naruto