Methylumbelliferyl acetate deacetylase

In enzymology, a methylumbelliferyl-acetate deacetylase (EC 3.1.1.56, esterase D) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are 4-methylumbelliferyl acetate and H₂O, whereas its two products are 4-methylumbelliferone and acetate.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is 4-methylumbelliferyl-acetate acylhydrolase. This enzyme is also called esterase D.