Methylenetetrahydrofolate dehydrogenase (NAD )

In enzymology, a methylenetetrahydrofolate dehydrogenase (NAD⁺) (EC 1.5.1.15) is an enzyme that catalyzes the chemical reaction

5,10-methylenetetrahydrofolate + NAD⁺ ⇌ 5,10-methenyltetrahydrofolate + NADH + H⁺

Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NAD⁺, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:NAD⁺ oxidoreductase. This enzyme is also called methylenetetrahydrofolate dehydrogenase (NAD⁺). This enzyme participates in one carbon pool by folate.