Manini Mishra is married to actor Mihir Mishra, who has appeared as Dr Rahul in the popular TV serial Sanjivani.
Manini Mishra was first recognized for playing Pari Kapadia, the best friend of villain Mallika, in the TV serial Jassi Jaissi Koi Nahin.
Mishra played Aunt Chanchal in the TV series Ghar Ki Lakshmi Betiyaan. In 2005, she had a part in The Great Indian Comedy Show of Star One, and was also in the reality show Nach Baliye with her husband. Manini Mishra played Ragini in SAB TV's serial Twinkle Beauty Parlour, and also appeared in the popular show Remix. Mishra has also appeared as Dr Sonali Barwe, a forensic expert, in the detective series C.I.D. from 2010 to 2011.
She also appeared in popular Sahara one drama Kesariya Balam Aavo Hamare Des.
In 2006, Mishra appeared in the film Krrish, as a friend of the character of Priyanka Chopra.
In 2008, Mishra appeared in the film Fashion as Sheena Bajaj, a fashion magazine expert.
In 2009-2010, she appeared in the youth oriented Disney show Kya Mast Hai Life. She portrayed the role of a Bollywood superstar mother, Sushmita Juneja, who cannot speak Hindi properly.
She also portrayed the role of Vijaya, elder sister of Sati, portrayed by Mouni Roy in mythological serial Devon Ke Dev – Mahadev.
From 2011, she is occasionally seen in courtroom drama Adaalat as prosecution lawyer "Devyani Bose".
She had featured in Student of the Year (film) where she played the role of Geetha,Abhimanyu(Siddharth Malhotra)'s bad aunt.
She was part of Shashi-Sumeet Mittal's popular show Diya Aur Baati Hum, aired on Star Plus and portrayed the character of inspector Singh. Of late she was in the star plus show pardes mein hai mera dil
Manini Mishra was one of the top ten finalists in the 1994 Miss India beauty pageant.