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Jill Bargonetti

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Jill Bargonetti


Jill Bargonetti Jill Bargonetti Wikipedia


Professor jill bargonetti pbs


Jill Bargonetti is an African-American and Italian-American scientist and an advocate of public higher education. Her higher education began in Bronx High School of Science, one of the accredited specialized high schools in NYC from which she went on to attend SUNY Purchase where she majored in biology and dance. She then received her Masters from New York University in 1987 and was accepted to the PhD program in NYU. She was one of 41 black students out of 3,288 scientists to receive a doctorate in biology in 1990. She went on to receive her post-doctoral training in Columbia University where she worked alongside Dr. Carol Prives and made the discovery of p53 and its functions in cancers. Her breakthrough research earned her the Presidential Early Career Award for Scientists and Engineers from William Clinton in 1997. While she is a leading scientist in the field of cancer research, she is also a dancer and a professor in the science at CUNY Hunter College and has been teaching since 1994.

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Dr jill bargonetti


Biography

Born on October 10, 1962 in New York Hospital, Jill Bargonetti is the daughter of Adah Askew and Arthur Bargonetti. Her mother and father are African-American and Italian respectively and they met in a club in Harlem where her father played the trumped in a band while her mother danced. Growing up in an interracial home as well as a multiracial community with whites, Asians, Hasidic Jews in the Upper West Side of Manhattan fostered diversity. Ultimately, this was a valuable experience and she believes it helped her to learn much. It is in this community that Bargonetti accredits that she discovered her passion for dancing. Her mother was her main source of inspiration for the arts as Askew studied dance at Hunter College under a leading anthropological African dancer by the name Pearl Primus. The close bond between Askew and Bargonetti is shown in the book Mothers and Daughters published in 1995 while also highlighting other mother-daughter pairs through essays, poetry and photographs.

Throughout her elementary education at Hunter College Elementary School and even high school education at the Bronx High School of Science, she danced and aspired to become a professional dancer. She completed her undergraduate education at State University of New York at Purchase College from where she received her bachelors in biology. Having still been passionate about dance, she commuted from Harlem to classes in order to be able to perform in Sounds of Motion, a dance company found by Dianne McIntyre. One of her biggest roles was dancing in a theater piece called “Takeoff From a Forced Landing” which strived to tell the story of support and independence. Bargonetti’s role in this play was a “strong-willed businesswoman” portraying “the loneliness and confusion such independence may create.” Prior to that, she studied at Alvin Ailey for two summers.

While she was devoted to the arts and found much joy in performing, she also developed a love and appreciation for the sciences. Continuing her education in the sciences, she went on to get Masters in biology from New York University in 1987 while simultaneously being a research technician and a teaching assistant there. She says of her career decision of pursuing biology and research: “To be honest, I still do not think I ever ‘decided’ to become a biologist. I simply found myself hooked on generating data and answering questions that had not been answered before.” Of her experience as a graduate student working as a research technician, she recalls being one of the few minority students. While having a supportive mentor and respective postdoctoral fellows, often times she was faced with racial stereotypes where she was questioned by several of the black clean up staff for wanting to enter the “white world” and occasionally being mistaken for the clean up staff herself during visits to her lab by other distinguished scientists

Following her completion of her Masters, Bargonetti went on to get her Ph.D. from New York University from 1987 – 1990. Her thesis paper focused on how to control DNA replication. From there, she went on to receive her post-doctoral training at Columbia University from 1990 – 1994 where she studied the tumor suppressor properties of the p53 gene and protein. Her discovery of the functions of the p53 protein was by accident as she initially used it as a control for one of her experiments aimed at studying a cancer-causing virus. However, it was then she stumbled upon the fact that p53 was really rather significant as it acted a transcription factor with the ability to specifically bind to the DNA and thus transcribe the genome. In her experiment, she found that the p53 protein could thus lead to downstream production of a viral protein which negatively regulated p53 and in effect, give damaged cells the advantage of proliferating out of control. She quickly tried to convince her lab members of her discovery and eventually published a paper.

Unfortunately, even during the time of her biggest discovery and one of the major contributions to science, she was still faced with racial discrimination. As she wrote a grant proposal for the Damon Runyon-Walter Winchell Cancer Fellowship, she was told that the grant she received would be one specifically geared towards minorities with the underlying assumption that that one was the least competitive. However, the fellowship itself was known to be prestigious and rather competitive. She was awarded the fellowship in 1991 and while she did not receive the fellowship geared toward minorities, she did get the Honorable Mention Award-NSF Postdoctoral Fellowship for Minorities.

Following her postdoctoral training, Bargonetti was offered an assistant professorship position at City University of New York at Hunter College, which she graciously accepted as it was known for being a minority institution with students primarily of black, Hispanic and Asian origin. The biological studies department at Hunter College is also part of the Research Centers in Minority Institution programs of the National Institutes of Health. Furthermore, in 1997, she was awarded the Presidential Early Career Award for Scientists and Engineers from William Clinton for “scholarly work in cancer related studies of cell growth and gene expression and involvement of undergraduate, graduate and especially minority students in the discovery process.” She became a full-time professor at Hunter College in 2007 and the Chair of the MCD PhD subprogram of the CUNY Graduate Center in 2009 to 2015.

She currently has her own laboratory located in the Belfer Research Building as part of the Weill Cornell Medical College and continues to teach at Hunter College. She is married to Nicholas Chavarria, a bilingual special education evaluator for the Board of Education and has two sons – Carlo and Milo, one dark and one blond, whom she uses to explain genetic phenomenon.

Bargonetti was featured on a PBS series called "American Graduate Day" in 2015. Additionally, she spoke at a TedX "Borders and Belonging" event at City University of New York (CUNY) in 2016.

Publications

  • Rosso M, Polotskaia A, Bargonetti J. Homozygous mdm2 SNP309 cancer cells with compromised transcriptional elongation at p53 target genes are sensitive to induction of p53-independent cell death. Oncotarget. 2015 Oct 27;6(33):34573-91. doi: 10.18632/oncotarget.5312. PMID 26416444
  • Pfister NT, Fomin V, Regunath K, Zhou JY, Zhou W, Silwal-Pandit L, Freed-Pastor WA, Laptenko O, Neo SP, Bargonetti J, Hoque M, Tian B, Gunaratne J, Engebraaten O, Manley JL, Børresen-Dale AL, Neilsen PM, Prives C. Mutant p53 cooperates with SWI/SNF chromatin remodeling complex to regulate VEGFR2 in breast cancer cells. Genes Dev. 2015 Jun 15;29(12):1298-315. doi: 10.1101/gad.263202.115. Epub 2015 Jun 16. PMID 26080815
  • Polotskaia, A., Xiao, G., Reynoso, K., Hendrickson, R., Martin, C., Qui, W. and J. Bargonetti. Proteome-wide Analysis of Mutant p53 Targets in Breast Cancer Identifies New Levels of Gain-of-Function that Influence PARP, PCNA and MCM4. (2015) Proc Natl Acad Sci U S A.;112(11):E1220-9.
  • Xiao, G., Kue, P., Bhosle and J. Bargonetti. Decarbamoyl Mitomycin C (DMC) Activates p53-independent Ataxia Telangiectasia and Rad3 Related Protein (ATR) Chromatin Eviction. (2015) Cell Cycle Epub ahead of print Jan 7. PMID 25565400
  • Shi, M., Shtraizent, N., Polotskaia, A., Bargonetti, J. H. Matsui. Impedimetric Detection of Mutant p53 Biomarker-Driven Metastatic Breast Cancers under Hyposmotic Pressure. (2014) PloS One Jan 7;9(6):e99351
  • Hoffman S., Martin, D., Melendez A. and J. Bargonetti C. elegans p53 and Beclin 1 are involved in DNA repair. (2014) PloS One Feb 20;9(2):e88828.
  • Shi, M., Shtraizent, N., Polotskaia, A., Bargonetti, J. H. Matsui. Impedimetric Detection of Mutant p53 Biomarker-Driven Metastatic Breast Cancers under Hyposmotic Pressure. (2014) PloS One Jan 7;9(6):e99351
  • Okoro D., Arva N., Gao, C., Polotskaia A., Puente, C., Rosso, M., and J. Bargonetti. Endogenous Human MDM2-C is Highly Expressed in Human Cancers and Functions as a p53-independent Growth Activator. (2013) PloS One Oct 11;8(10):e77643.
  • Catalina-Rodriguez, O., Preet, A., Kolukula, V., Furth, P., Albanese, C, Bargonetti, J. and M.L. Avantaggiati. Dietary regulation of p53 mutant levels influences tumorigenesis. (2012) Cell Cycle. 2012 Nov 14;11(23)
  • Polotskaia, A., Krett, N., Shanmugam, M., Gamss, S., Rosen, S., and Bargonetti J. 8-Aminoadenosine activates p53-independent cell death of metastatic breast cancers. (2012) Molecular Cancer Therapeutics.
  • Okoro D., Rosso M., and J. Bargonetti. Splicing up Mdm2 for Cancer Proteome Diversity. Genes & Cancer August 2012.
  • Freed-Pastor, W. A., Mizuno, H., Zhao, X., Langerod, A., Moon, S.-H., Rodriguez-Barrueco, R., Barsotti, A., Chicas, A., Li, W., Polotskaia, A., Bissell, M. J., Osborne, T. F., Tian, B., Lowe, S. W., Silva, J. M., Borrensen-Dale, A.-L., J., L. A., Bargonetti, J., and Prives, C. (2012) Mutant p53 Disrupts Mammary Acinar Morphogenesis via the Mevalonate Pathway, Cell 148(1-2):244-58.
  • Success in Molecular Genetics: The Pink Flower" in Voices of Black American Pioneers, edited by Vernon Farmer, Greenwood Publishing Group, Westport, Connecticut (2012).
  • Brekman, A., Singh K.E., Polotskaiai A., Kundu N. and Bargonetti J. A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation. (2011) Breast Cancer Res. 13 (1):R3
  • Boamah, E.K., Brekman, A., Tomasz, M.., Myeku, N., Figueiredo-Pereira, M., Hunter, S., Meyer, J. Bhosle, R.C. and Bargonetti, J. DNA adducts of decarbamoyl mitomycin C efficiently kill cells without wild-type p53 resulting from proteasome-mediated degradation of Checkpoint Protein 1. (2010) Chem. Res. Toxicol. 19 (23): 1151-62
  • Bargonetti, J., Champeil E. and Tomasz, M. Differential Toxicity of DNA Adducts of Mitomycin C. (2010) Invited Review Journal of Nucleic Acids. Jul 29;2010. pii: 698960
  • Paz, M.M., Ladwa, S., Champell, E., Liu, Y., Rockwell, S. Boamah, E.K., Bargonetti, J., Callahan, J., Roach, J., and Tomasz, M. Mapping DNA Adducts of Mitomycin and Decarbamoyl Mitomycin C in cell Lines Using Liquid Chromatography/ Electrospray Tandem Mass Spectrometry. (2008) Chem. Res. Toxicol., 21(12): 2370-2378.
  • Arva, N., Talbott, K., Okoro, D., Brekman, A., Qiu, W., and Bargonetti, J. Disruption of the p53-Mdm2 Complex by Nutlin-3 Reveals Different Cancer Cell Phenotypes. (2008) Ethnicity and Disease, 18(2 Suppl 2):S2-1-8.
  • Boamah EK, White DE, Talbott KE, Arva NC, Berman D, Tomasz M, Bargonetti J. Mitomycin-DNA adducts induce p53-dependent and p53-independent cell death pathways. ACS Chem Biol. 2007 Jun 15;2(6):399-407. Epub 2007 May 25.
  • White DE, Talbott KE, Arva NC, Bargonetti J. Mouse double minute 2 associates with chromatin in the presence of p53 and is released to facilitate activation of transcription. Cancer Res. 2006 Apr 1;66(7):3463-70.
  • Hui L, Zheng Y, Yan Y, Bargonetti J, Foster DA. Mutant p53 in MDA-MB-231 breast cancer cells is stabilized by elevated phospholipase D activity and contributes to survival signals generated by phospholipase D. Oncogene. 2006 Nov 23;25(55):7305-10. Epub 2006 Jun 19
  • Arva, N.C., Gopen, T.R., Talbott, K.E., Campbell, L.E., Chicas, A., White, D.E., Bond, G., Levine, A. and J. Bargonetti (2005) A chromatin associated and transcriptionally inactive p53-mdm2 complex occurs in mdm2 SNP309 homozygous cells. J. Biol. Chem. 280(29):26776-87
  • Bond, G.L., W. Hu, E.E. Bond, H. Robins, F. Bartel, H. Taubert, P. Wuerl, K. Onel, L. Yip, S. Hwang, L.C. Strong, N.C. Arva, J. Bargonetti, G. Lozano, and A.J. Levine (2004) A Single Nucleotide Polymorphism in the Mdm2 Promoter Attenuates the p53 Tumor Suppressor Pathway and Accelerates Tumor Formation in Humans. Cell 119:591-602.
  • Abbas, T., D. White, L.Hui, .D.A., Foster and J. Bargonetti (2004) Inhibition of p53 transcription by down-regulation of protein kinase C delta. Journal of Biological Chemistry 279 (11):9970-9977
  • Hui, L., Abbas, T., Bargonetti, J., and D.A. Foster. (2004). Phospholipase D Elevates the Level of MDM2 and Suppresses DNA Damage-Induced Increases in p53. Mol. Cell Biology (24): 5677-5686.
  • Molina, M. P., C. Cain, and J. Bargonetti (2003) In Vivo footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability. Methods in Molecular Biology 234:151-70
  • Abbas, T., M. Olivier, J. Lopez, S. Houser, G. Xiao, G. S. Kumar, M. Tomasz, and J. Bargonetti (2002). Differential activation of p53 by the various adducts of Mitomycin C. Journal of Biological Chemistry 277(43):40513-9.
  • Bargonetti, J. and J.J. Manfredi. (2002). Multiple roles of the tumor suppressor p53. Curr. Opin. Oncology. 14:86-91.
  • Houser, S., S.Koshlatyi, T. Lu, T. Gopen, and J. Bargonetti (2001). Camptothecin and Zeocin Can Differentially Increase p53 Levels During all Cell Cycle Stages. Biochem Biophys Res Commun. 289:998-1009.
  • Chicas, A., P. Molina, and J. Bargonetti (2000). Mutant p53 forms a complex with Sp1 on HIV-LTR DNA. Biochem Biophys Res Commun. 279:383-390.
  • Xiao, G., A. Chicas, M. Olivier, Y. Taya, S. Tyagi, F.R. Kramer and J. Bargonetti, (2000). p53 requires a damage signal to activate gadd45. Cancer Research 60: 1711-1719.
  • Boydston-White, S., T. Gopen, S. Houser, J. Bargonetti and M. Diem, (1999). Infrared spectroscopy of human tissue: V. Infrared Spectroscopic studies of myeloid leukemia (ML-1) cells at different phases of the cell cycle. Biospectroscopy 5: 219-227.
  • Xiao, G., D. White, and J. Bargonetti (1998). p53 binds to a constitutively nucleosome free region of the mdm2 gene. Oncogene 16:1171-1181.
  • Bargonetti, J., A. Chicas, D. White, and C. Prives (1997). p53 represses Sp1 DNA Binding and HIV-LTR directed transcription. Cellular & Molecular Biology 43:935-949.
  • Chen, X., J. Bargonetti, and C. Prives, (1995). p53, through p 21 (WAF1/CIP1), induces cyclin D1 synthesis. Cancer Research 55:4257-4263.
  • Prives, C., J. Bargonetti, G. Farmer, E. Ferrari, P. Friedlander, U. Hubsher, L. Jayaraman, N. Pavletich, and Y. Wang, (1994). The DNA binding properties of the p53 tumor suppressor protein. CSHS on Quan. Bio. LIX:207-213.
  • Bargonetti, J., J.J. Manfredi, X. Chen, D.R. Marshak, and C. Prives, (1993). A proteolytic fragment from the central region of p53 has marked sequence-specific binding activity when generated from wild-type but not from oncogenic mutant p53 protein. Genes and Dev. 7:2565-2574.
  • Bargonetti, J., P.Z. Wang, and R.P. Novick, (1993). Measurement of gene expression by translational coupling: effect of copy mutations on pT181 initiator synthesis. EMBO 12:3659-3667.
  • Friedman, P.N., X. Chen, J. Bargonetti, and C. Prives, (1993). The p53 protein is an unusually shaped tetramer that binds directly to DNA. Proc. Natl. Acad. Sci. USA. 90:3319-3323.
  • Bargonetti, J., I. Reynisdottir, P.N. Friedman, and C. Prives, (1992). Site-specific binding of wild-type p53 to cellular DNA is inhibited by SV40 T antigen and mutant p53. Genes and Dev. 6:1886-1898.
  • Farmer, G., J. Bargonetti, H. Zhu, P. Friedman, R. Prywes, and C. Prives, (1992). Wild-type p53 activates transcription in vitro. Nature 358:83-86.
  • Zambetti, G.P., J. Bargonetti, K. Walker, C. Prives, and A.J. Levine, (1992). Wild-type p53 mediates positive regulation of gene expression through a specific DNA sequence element. Genes and Dev. 6:1143-1152.
  • Prives, C., J. Bargonetti, P.N. Friedman, J.J. Manfredi, and E.H. Wang, (1991). Functional consequences of the interactions of the p53 tumor suppressor protein and SV40 large tumor antigen. CSHS on Quan. Bio. LVL:227-235.
  • Bargonetti, J., P.N. Friedman, S.E. Kern, B. Vogelstein, and C. Prives, (1991). Wild-type but not mutant p53 immunopurified proteins bind to sequences adjacent to the SV40 origin of replication. Cell 65:1083-1091
  • References

    Jill Bargonetti Wikipedia