Inosinate nucleosidase

In enzymology, an inosinate nucleosidase (EC 3.2.2.12) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are 5'-inosinate and H₂O, whereas its two products are D-ribose 5-phosphate and hypoxanthine.

This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is 5'-inosinate phosphoribohydrolase. This enzyme participates in purine metabolism.