Hydroxybutyrate dimer hydrolase

In enzymology, a hydroxybutyrate-dimer hydrolase (EC 3.1.1.22) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are (R)-3-((R)-3-hydroxybutanoyloxy)butanoate and H₂O, whereas its product is (R)-3-hydroxybutanoate.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is (R)-3-((R)-3-hydroxybutanoyloxy)butanoate hydroxybutanoylhydrolase. This enzyme is also called D-(−)-3-hydroxybutyrate-dimer hydrolase. This enzyme participates in butanoate metabolism.