Holub is a surname of Czech origin (it means pigeon).
It may refer to:
Allen I. Holub, computer scientist
Beata Holub, Polish high jumper
Emil Holub, Czech doctor, explorer, cartographer, and ethnographer in Africa
E. J. Holub, AFL and NFL player
Josef Ludwig Holub - botanist
Miroslav Holub, Czech poet and immunologist
Radim Holub, Czech footballer
Renate Holub, political philosopher and social theorist
Robert C. Holub, 28th chancellor of the University of Massachusetts Amherst
Eva Holubová (born 1959), a Czech actress