Guanosine 3',5' bis(diphosphate) 3' diphosphatase

In enzymology, a guanosine-3',5'-bis(diphosphate) 3'-diphosphatase (EC 3.1.7.2) is an enzyme that catalyzes the chemical reaction

guanosine 3',5'-bis(diphosphate) + H₂O ⇌ guanosine 5'-diphosphate + diphosphate

Thus, the two substrates of this enzyme are guanosine 3',5'-bis(diphosphate) and H₂O, whereas its two products are guanosine 5'-diphosphate and diphosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on diphosphoric monoester bonds. The systematic name of this enzyme class is guanosine-3',5'-bis(diphosphate) 3'-diphosphohydrolase. Other names in common use include guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase, PpGpp-3'-pyrophosphohydrolase, and PpGpp phosphohydrolase. This enzyme participates in purine metabolism.