Gamma glutamyl gamma aminobutyrate hydrolase

In enzymology, a gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) is an enzyme that catalyzes the chemical reaction

4-(gamma-glutamylamino)butanoate + H₂O ⇌ 4-aminobutanoate + L-glutamate

Thus, the two substrates of this enzyme are 4-(gamma-glutamylamino)butanoate and H₂O, whereas its two products are 4-aminobutanoate and L-glutamate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 4-(gamma-glutamylamino)butanoate amidohydrolase. Other names in common use include gamma-glutamyl-GABA hydrolase, PuuD, and YcjL. This enzyme participates in urea cycle and metabolism of amino groups.