Formyltetrahydrofolate deformylase

In enzymology, a formyltetrahydrofolate deformylase (EC 3.5.1.10) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are 10-formyltetrahydrofolate and H₂O, whereas its two products are formate and tetrahydrofolate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 10-formyltetrahydrofolate amidohydrolase. This enzyme participates in glyoxylate and dicarboxylate metabolism and one carbon pool by folate.