From 2001, Wisbey collaborated with Jonny Trunk, founder of Trunk Records, to produce the album Dirty Fan Male, based on Trunk's experiences organising glamour models' fan clubs. The album comprises Wisbey's amusing recitals of the fan mail they received and it was later turned into an acclaimed live show and a book.
The inclusion of Wisbey's song "The Ladies' Bras" on the 2007 compilation album Now We Are Ten and its appearances on The All Day Breakfast Show proved so successful that it was released as a single and became the shortest single ever to enter the UK Singles Chart following a campaign on BBC Radio 1 by Scott Mills. At 36 seconds long, it became the shortest song ever to make the UK Singles Chart, reaching number 27 in September 2007.
Wisbey has also appeared as an actor in several Doctor Who audio adventures, made by Big Finish Productions.
Wisbey narrated the short film Corridors directed by Chris Devey
He also voiced Perky in The Pinky and Perky Show.
In television, Wisbey performed in and contributed to the writing of Alistair McGowan's Big Impression. He is also the voice of the character Migo in The Roly Mo Show. In 2005 he appeared as himself in the documentary Dirty Fan Male about his live comedy show of the same name. Other parts have included appearances in the series Peak Practice and The Bill.
In radio, Wisbey has appeared in the BBC productions Trapped, Undone, The Secret World and Go 4 It. Wisbey also appeared in the fifth instalment of the popular series Wrong Turn 5: Bloodlines, as a character named "Mose". Wisbey also writes for and performs in Dead Ringers (especially the musical items)
Wisbey is currently appearing as 'Doctor Brain' in the CBBC series Ultimate Brain
He also voices several characters in the Cbeebies TV show Tree Fu Tom
From 2002, Wisbey contributed to the writing of stage versions of the Dick Barton adventure serial, which he also performed in. He is the author of the book Young Dick Barton - The Making of a Legend.
In the theatre, Wisbey has worked closely as a musical director, arranger, and sometimes composer alongside the award-winning director, Rufus Norris, on three productions for the Young Vic Theatre Company. The first, Sleeping Beauty, ran at the Young Vic itself in 2002, then transferred to the Barbican and then to the New Victory on Broadway. Hergé's Adventures of Tintin premiered at the Barbican two years later, followed by a tour and West End run in 2007. The other production was the World Premier of Tanya Ronder's acclaimed adaptation of the book Vernon God Little. Recently Duncan Wisbey became noticed on Radio 1 with the U.K's shortest song to reach the U.K top 40 with the song "The Ladies' Bras" which was 36 seconds long and reached number 27 in U.K top 40.