Diguanidinobutanase

In enzymology, a diguanidinobutanase (EC 3.5.3.20) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are 1,4-diguanidinobutane and H₂O, whereas its two products are agmatine and urea.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is 1,4-diguanidinobutane amidinohydrolase.