In enzymology, a deoxynucleotide 3'-phosphatase (EC 126.96.36.199) is an enzyme that catalyzes the chemical reaction
a deoxynucleoside 3'-phosphate + H2
a deoxynucleoside + phosphate
Thus, the two substrates of this enzyme are deoxynucleoside 3'-phosphate and H2O, whereas its two products are deoxynucleoside and phosphate.
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is deoxyribonucleotide 3'-phosphohydrolase. Other names in common use include 3'-deoxynucleotidase, and 3'-deoxyribonucleotidase.