Deoxynucleotide 3' phosphatase

In enzymology, a deoxynucleotide 3'-phosphatase (EC 3.1.3.34) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are deoxynucleoside 3'-phosphate and H₂O, whereas its two products are deoxynucleoside and phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is deoxyribonucleotide 3'-phosphohydrolase. Other names in common use include 3'-deoxynucleotidase, and 3'-deoxyribonucleotidase.